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For the highly active α-chloroacetamide ( 7 ), O-to-S exchange (resulting in α-chlorothioacetamide 9 ) improved JAK3 potency (30.3 ± 11.2 nM versus 18.9 ± 4.7 nM, respectively) while selectivity over BTK was not significantly altered, as reflected by Δ p IC 50 JAK3/BTK values of 0.75 for probe 7 and 0.46 for probe 9