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3a–d, IR treatment led to a significant increase in the content of cellular lipid ROS, as determined by flow cytometry using the fluorescent probe C11-BODIPY, a membrane-targeted lipid sensor dye, and knocking down HDAC4 resulted in an increase in the lipid ROS content, indicating that under IR treatment, HDAC4 knockdown substantially increased the IR-induced accumulation of lipid ROS
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